Abstract Homology-directed repair-mediated knock-in (HDR-KI) in combination with CRISPR-Cas9-mediated double strand break (DSB) leads to high frequency of site-specific HDR-KI. While this characteristic is advantageous for generating genetically modified cellular and animal models. HDR-KI efficiency in mammalian cells remains low. Since avian DT40 cells offer distinct advantage of hig... https://www.remtavares.com/