Upcoming, we treated cells with distinctive concentrations of NAD+. When included for 24 h at 80 µM in HeLa and 400 µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Extended Data Fig. 1k,l). Furthermore, 16 µM of NAD+ was sufficient to lower fork velocity by ~forty% in HeLa cells https://nad-and-its-effect-on-dna23578.aboutyoublog.com/34096491/5-simple-statements-about-is-nad-important-for-cognitive-function-explained