The lab claims fixation will induce the dyes to leak in the cells. I feel the belief is always that fixation may well problems cell membranes. I did look for a paper which describes fixation using methanol or acetic acid, soon after staining using a DCFDA spinoff. DCFDA could be https://2-fdckhcl35790.blogrelation.com/35408977/how-much-you-need-to-expect-you-ll-pay-for-a-good-2-fdck-hcl